Journal: Current Environmental Health Reports

Article Title: Cannabis Exposure During Critical Windows of Development: Epigenetic and Molecular Pathways Implicated in Neuropsychiatric Disease

doi: 10.1007/s40572-020-00275-4

Figure Lengend Snippet: Summary of studies (from the past 10 years) on the effect of pre-gestational and prenatal cannabis exposure on genes and molecular pathways in humans. Studies are in reverse order chronologically by publication year

Article Snippet: Fransquet et al. 2017 [ •] , Nested case-control study n = 804 maternal subjects; 44 cannabis users anytime during pregnancy and 760 non-user controls , Prenatal , Buccal cells of neonates of maternal subjects F1 generation , DNA methylation SEQUENOM MassARRAY , • Gestational cannabis use associated with increased methylation at one CpG site tested in DRD4 (CpG.21.22.2) (β + 1.48, 95% CI: 0.02–2.93, p = 0.047). • At CpG.32, weak evidence that gestational cannabis is associated with increased methylation when adjusting for other substance use (β + .67, 95% CI: − 0.12-1.46, p = 0.09). • No associations remained significant after correction for multiple testing using a Bonferroni corrected significance level of 0.0026 given the 19 CpG units examined. , • DRD4 plays key role in dopamine signaling and is associated with drug use. • Increased DRD4 methylation in saliva has been associated with increased severity of ADHD symptoms in children. • Tissue specificity (using buccal cells instead of brain tissue) could have contributed to the null findings..

Techniques: Cannabis, DNA Methylation Assay, Expressing, Methylation, Immunoprecipitation, Methylated DNA Immunoprecipitation, Control, Methylation Sequencing, Membrane, Gene Expression

Journal: Current Environmental Health Reports

Article Title: Cannabis Exposure During Critical Windows of Development: Epigenetic and Molecular Pathways Implicated in Neuropsychiatric Disease

doi: 10.1007/s40572-020-00275-4

Figure Lengend Snippet: Summary of studies (from the past 10 years) on the effect of pre-gestational, prenatal, and adolescent cannabis exposure on genes and molecular pathways in animals. Studies are in reverse order chronologically by publication year

Article Snippet: Fransquet et al. 2017 [ •] , Nested case-control study n = 804 maternal subjects; 44 cannabis users anytime during pregnancy and 760 non-user controls , Prenatal , Buccal cells of neonates of maternal subjects F1 generation , DNA methylation SEQUENOM MassARRAY , • Gestational cannabis use associated with increased methylation at one CpG site tested in DRD4 (CpG.21.22.2) (β + 1.48, 95% CI: 0.02–2.93, p = 0.047). • At CpG.32, weak evidence that gestational cannabis is associated with increased methylation when adjusting for other substance use (β + .67, 95% CI: − 0.12-1.46, p = 0.09). • No associations remained significant after correction for multiple testing using a Bonferroni corrected significance level of 0.0026 given the 19 CpG units examined. , • DRD4 plays key role in dopamine signaling and is associated with drug use. • Increased DRD4 methylation in saliva has been associated with increased severity of ADHD symptoms in children. • Tissue specificity (using buccal cells instead of brain tissue) could have contributed to the null findings..

Techniques: Cannabis, Animal Model, DNA Methylation Assay, Methylated DNA Immunoprecipitation, Immunoprecipitation, Methylation, Expressing, Gene Expression, Mouse Assay, Enzyme-linked Immunosorbent Assay, Control, Modification, Functional Assay, Disruption, Activity Assay, Methylation Sequencing, Virus, Quantitative Proteomics, Cell Function Assay, Comparison, Membrane, Transmission Assay, Binding Assay, Inhibition, Chromatin Immunoprecipitation